Journal of The Showa Medical Association
Online ISSN : 2185-0976
Print ISSN : 0037-4342
ISSN-L : 0037-4342
PATHOHISTOLOGICAL STUDIES ON THE HEPATIC CHANGES OF RATS FED 2-ACETYLAMINOFLUORENE, ESPECIALLY ON THE HISTOGENESIS OF CYSTOADENOMA
Fumiaki Sagawa
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1959 Volume 19 Issue 1 Pages 44-59

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Abstract
The early pathological and histological changes in liver of wistar rats fed with 0.03% 2-acetylaminofluorene (2-A.A.F.) were studied. A few observations were made on the cystoadenomatous formation and on the relation between liver cells and epithelial cells in the bile duct.
1) The small clear cells (epithelial cells originated from small bile duct) were found around the portal area at the 7th day following the administration. The dendric proliferation of these cells was seen in the space of liver cell trabeculae with the elapse of time. It was found that the periphery of the liver lobules was surrounded by small clear cells and the psudo-annular cirrhosis in liver after one month following the adminstration. At this stage some of the small clear cells have changed into cystoadenoma.
2) The mode of development of cystoadenoma was as follows. The stagnation of the secret was occurred, as the result of stenosis in the lumen of ductlike structure of small clear cells, thus the cystic dilatation was revealed as cystoadenoma.
The size and form of cystoadenoma are rich in variety. The form of epithelial cells lining the cyst was flat, cuboidal, cylindrical and so on.
3) On the 31st day following the administration marked progressive or hyperplastic changes of the liver cells were observed. This change indicates the nodular hyperpaasia.
4) These small clear cells were stained differently from the liver cells or the epithelial cells in the bile duct by PAS-methylgreen staining. The cytoplasm of non-progressive liver cells contained PAS-positive large granules abundantly. PAS-positive granules in the progressive liver cells were small and scatteredly (prominent decrease in the number of granules) . The transformation and decrease in the number of mitochondriae were found remarkably by Heidenhain's iron haematoxylin and Altmann's stain. PAS-positive granules were not found in the cytoplasm of epithelial cells in the small clear cell. The nuclei and cell membrane of these cells were stained green with methylgreen. The granules of mitochondria did not noticed with Heidenhain's iron haematoxylin and Altmann's stain.
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