Abstract
By repeated painting of 3-methylchoranthrene (as 1.5% benzene solution) to the skin of d d strain mice about 15 times (twice a week for 5 weeks and then once a week for ca. 5-6 weeks), transplantable generalized lymph nodes swelling in mice with leukemic change in the peripheral blood were obtained.
1. On about 100 days following the administration in 8 cases out of 60 mice, the generalized lymph nodes swelling (lymphomatosis Mider, G. B., et Morton, J. J., 1939) was observed. At the same time, when the lymphomatosis occurred, these mice suffered with acute leukocytosis, which was examined hemocytologically as well as pathohistologically. This is the acute leukemic lymphadenosis by 3-methylchoranthrene administration.
2. Successive transplantations have been made until 14 generations by the transplantation of the tissue of the swollen lymph nodes into the subcutaneous tissue of the same strain mice. However, they died by the epidemic pneumonia in the 14 generation unfortunately.
The ratio of transplantability was about 400 (68 cases out of 180 mice) . The transplanted tumor grew up rather rapidly at the site of inoculation expansively with the tendency of infiltration to the surrounding tissue. Tumor-bearing mice died by cachexia.
Leukemic changes were observed in 4 cases out of 68 mice in which the transplantation succeeded. It is very curious that these 4 cases did ont show marked swelling of the lymph nodes, the spleen, the liver and the kidneys, although the pathohitological features were quite the same as above-mentioned findings. When and how the transplanted mice obtained the leukemic phase will be the theme for the further study.
Transplantable leukemic lymphadenosis (chiefly as subcutaneous tumor in transplanted mice, but sometimes as lymphatic leukemic) obtained relatively easily by the administration of 3-methylchoranthrene.
