ON ISONICOTINIC ACID HYDRAZID RESISTANT TUBERCLE BACILLI

Report 1. Studies on the cultivation of isonicotiníc acid hydrazide resisiant tubercle bacilli

Abstract

1) Stock cultured strain of Mycobacterium tuberculosis var, homnis was isolated from sputum. The sensitive or resistant strain to INH, were treated with 4% NaOH and then inoculated on the media containing 3% KH₂PO₄. The number of colonies observed was not related to the sensitivity or the grade of resistance of the strains. The same result was obtained in case of 4% H₂SO₄ treatment, but their population increased when the latter treatment was employed.
2) The strains and sputum were treated with 4% NaOH and were inoculated on the pyruvic acid containing media and the modified III Kirchner agar media. The colonial populations on pyruvic acid containing media were generally greater than those on 3% KH₂PO₄ media. The populations on the modified Kirchner agar media were about the same as those on 3% KH₂PO₄ media.