ON ISONICOTINIC ACID HYDRAZID RESISTANT TUBERCLE BACILLI

Report 2. Change of the value of resistance

Abstract

1) The resistivity to INH of the INH resistant bacteria which isolated from sputum were examined in several generation. Their resistivity varied.
2) INH resistant strains and sputum were used. They were inoculated directly or after neutralization of the sputum or the treatment with 4% NaOH or 4% H₂SO₄ on the INH resistance testing media, consisted of 1% KH₂PO₄ media, 3% KH₂PO₄ media and 3% Na₂HPO₄ media. The highest resistance values were obtained on 3% Na₂HPO₄ (pH after inoculation: 6.8) .
3) The sputum was inoculated on the resistance testing media, consisted of 1% KH₂PO₄ media, 3% KH₂PO₄ media and 3% Na₂HPO₄ media. The resistance decreased in the order of 1% KH₂PO₄ media (pH 7.0), 3% KH₂PO₄ media (pH 6.4) and 3% Na₂HPO₄ media (pH 7.9) .
4) The sputum was cultured on resistance testing media, consisted of 3% KH₂PO₄ media and modifyed III Kirchner agar media. The higher resistance values were obtained on the modifyed III Kirchner agar media. Non of the strains with the highest resistance grew only in the modifyed Kirchner media.