Abstract
As stated in the previous report, acriflavine attenuated strain was found to have completely lost its pathogenicity in mice when inoculated subcutaneously or by lip scarification. Protection tests were attempted accoringly by the use of this strain on the development of listerial encephalitis by the infection with Listeria monocytogenes.
1. On investigating the route gf infection on mice, subcutaneous inoculation and lip scarification were found to give the best protection while intravenous, intracerebral and intraperitoneal inoculation, eye instillation and per os feeding were found to be insufficient. For practical purpose, subcutaneous inoculation is recommended.
2. A single subcutaneous inoculation of 0.5 mg of the attenuated bacilli into goats could prevent the development of listerial encephalitis.
3. The more the amount inoculated and the more the number of inoculations repeated, the stronger was the prophylactic effect against the development of listerial encephalitis in goats.
