Abstract
Stated in the following are the results of the experimental observation made on the normal growth and on the influence of physical factors and several anti-fungal preparations on the growth of M. gypseum by the use of time-lapse cinemicrographic technique.
1. Processes of budding from the spore, elongation and growth of the mycelium, and growth of the chramidspore as well as the nodular organ were recorded successfully.
2. Granular substance contained in the spore, mycelium, chramidspore and the nodular organ was found to have motility which was the morphological indication of the life of cells.
3. The curling effect observed on the mycelium by the addition of Griseofulvin was first observed on the terminal end of the mycelium and then there was the appearance of numerous curled branches. This phenomenon was characteristic to Griseofulvin.
4. The presence of curling factor, namely, Griseofulvin, was proved in the serum of individuals administered of Griseofulvin, in the content of the cantharidin bulla and also in the horny layer of the skin.
5. The influence of Griseofulvin on M. gypseum was confirmed to be fungistatic, while that of Variotin and Merthiolate was fungicidal.
6. Availing the technique of cinemicrography, it has become possible to prove the presence of an extremely small amount of Griseofulvin.
7. Mode of the appearance of curling effect was different depending on the concentration of Griseofulvin present and an observation period of 2-4 hours could telll the concentration of Griseofulvin in the fluid examined.
