Abstract
The experiments were performed to observe the effects of metabolic change of fat with the various levels of intake upon the vitamin B2 deficiency in the rats.
The rats, which were divided into three different groups group with each of high, medium and low fat contained diets), were fed with a vitamin B2 deficient diets. Change of weight, vitamin B2 and its ester type in a whole blood, total lipids, total cholesterol, trigly cerides, phospholipids, free fatty acids, and peroxidized lipids in sera were observed.
In the experiment of“recovery phase”, followed by the feeding of vitamin B2 deficient diet, the rats were divided into three groups with administration of free vitamin B2, vitamin B2-tetrabutylate daily and also in one large dose every four days respectively. Changes and improvements in weight, signs of vitamin B2 deficiency, lipid metabolism were compared.
The following results were obtained. The deterioration of clinical signs with vitamin B2 deficiency appeared much earlier and severely as the fatty level of given diet increased, while vitamin B2 level in whole blood decreased more remarkedly. Vitamin B2-tetrabutylate seemed much effective as compared with free vitamin B2 for the recovery both in clinical and biochemical aspects.
