In the neonates delivered by normal vaginal deliveries or caesarean section under anesthesia, the acid-base balance was studied with Astrup-micro-apparatus. Furthermore, the uterine regional blood flow was measured in nonpregnant and pregnant rabbits, using thermal flowmeters:
1) Metabolic acidosis of the mother was reflected on the neonate in both groups of the normal vaginal delivery and the caesarean section.
2) PO₂ of the neonate delivered by the normal vaginal delivery showed higher value compared against those of the caesarean section and this difference was significant statistically (P<0.001) .
3) For anesthesia for the caesarean section:
i) Those which would induce depression of blood pressure and/or peripheral vasoconstriction in the mother, such as spinal anesthesia and cyclopropane anesthesia, reduced PO₂ of the neonate.
ii) In case of other inhalation anesthetics, there were not any particular relationships between the kind of anesthetics and PO₂ of the neonate, insofar as the blood pressure of the mother was maintained at the normal level; it was concluded that exchange from nitrous-oxide-oxygen mixture to pure oxygen inhalation was needless after laparotomy.
4) No correlation was discernible between PO₂ of the neonate and Apgar score.
5) No particular aggravation of acid-base balance was recognized in cases of the emergency caesarean section compared with the elective one.
6) Bathing of the neonate after delivery improved respiration, metabolic acidosis, general condition, and peripheral circulation.
7) In the rabbit, hyperventilation caused a moderate decrease of uterine blood flow.

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The experiments were performed to observe the effects of metabolic change of fat with the various levels of intake upon the vitamin B₂ deficiency in the rats.
The rats, which were divided into three different groups group with each of high, medium and low fat contained diets), were fed with a vitamin B₂ deficient diets. Change of weight, vitamin B₂ and its ester type in a whole blood, total lipids, total cholesterol, trigly cerides, phospholipids, free fatty acids, and peroxidized lipids in sera were observed.
In the experiment of“recovery phase”, followed by the feeding of vitamin B₂ deficient diet, the rats were divided into three groups with administration of free vitamin B₂, vitamin B₂-tetrabutylate daily and also in one large dose every four days respectively. Changes and improvements in weight, signs of vitamin B₂ deficiency, lipid metabolism were compared.
The following results were obtained. The deterioration of clinical signs with vitamin B₂ deficiency appeared much earlier and severely as the fatty level of given diet increased, while vitamin B₂ level in whole blood decreased more remarkedly. Vitamin B₂-tetrabutylate seemed much effective as compared with free vitamin B₂ for the recovery both in clinical and biochemical aspects.

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An adequate treatment, such as iron for iron deficiency anemia or vitamin B12 and folic acid for pernicious anemia, has not been found for aplastic anemia. We assumed that erythropoietin might be a subunit of a serum apo-ceruloplasmin-like protein.
The result of our clinical data on the treatment of aplastic anemia by cerulopasmin was reported. Seventeen aplastic anemia patients were administrated separately or semultaneously with hog ceruloplasmin intramusculary or with floic acid parenterally. In some cases adrenal steroid hormone was given for their haemorrhagic diathesis.
The following results were obtained by the bone marrow, peripheral blood examination and the determination of serum iron, copper and ceruloplasmin.
(1) The combined application of ceruloplasmin and folic acid was the most effective for the treatment of aplastic anemia, especially that of maturation arrest type. This results agreed with the animal experimental data which showed the increase of erythropoietic substance in peripheral blood with combined administration of these drugs.
(2) In bone marrow, the increase of nucleated cells and polychromatic erythroblast was remarkable. The number of sideroblast decreased. In some cases hemoglobin remains in its high concentration which increased with the cell counts. In other cases hemoglobin increase delayed to the cell number.
(3) In most cases the serum iron level which was above 200 μg/dl remained in the same for one to two years after the remission when the treatment was terminated. The serum transferrin and the unsaturated iron binding capacity increased during the therapy.
(4) According to our method, the normal serum ceruloplasmin value is about 22-30 mg/dl. When it was under the normal level (about 10 mg/dl) the remission delayed. When it was over the normal level (about 30 mg/dl) prognosis is favourable. The normal ratio of serum copper to copper in ceruloplasmin is 90 %, in case of aplastic anemia it was 40-60 % and it returned to normal level after remission.
(5) No therapeutic effect was observed in aplastic type, when the disease was classified into four groups-aplastic type, hypoplastic type, normal type, and hyperplastic type.
The treatment was critisized as effective in cases who maintained the level over 3 million/cmm in red cell count and above 90 % in hemoglobin for more than one year without blood transfusion, and kept good condition in clinical manifestations such as haemorrhage, fever and so on.
This treatment is considered to be desirable for aplastic anemia, if the fetal emergency of the rapid decrease of leucocytes or platelets count could be avoided.

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By digesting the high antigenic yeast cytochrome c with chymotrypsin and cutting peptide chain stepwise, hypomolecular digested cytochrome c was obtained. The inhibiting effect of this substance against in vitro and in vivo anaphylactic reaction was tested in the guinea pig and the following results were obtained.
1. The sensitizing activity was found to be existing in cytochrome c digested within for 2 hrs, but it decreased remarkably in the material digested for over 3 hrs and by 15 hrs digestion it was lost completely.
2. The desensitzing activity of digested cytochrome c was tested in the guinea pig sensitized with intact cytochrome c. The desensitizing activity was demonstrated in the cases treated with cytochrome c digested for 3 hrs and 9 hrs. In the guinea pig desensitized with digested cytochrome c anaphylactic shock and Schultz-Dale reaction by intact cytochrome c were clearly inhibited.
3. Indicating I¹³¹labeled growth hormone and insulin, cytochrome c digested for 3 hrs and 9 hrs were fractionated with column chromatography using Sephadex 'G-50. Consequently, the main fraction was consisting of undigested cytochrome c, having molecular weight of over 25, 000 and halfway- digested cytochrome c, having molecular weight of about 6, 000. The inhibiting action of this fraction against anaphylaxis with undigested cytochrome c was also demonstrated.

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