STUDIES ON THE YEAST CYTOCHROME C ANAPHYLAXIS ON THE INHIBITIORY EFFECT OF DISRUPTED SUBSTANCE OF CYTOCHROME C TO ANAPHYLAXIS

Abstract

By digesting the high antigenic yeast cytochrome c with chymotrypsin and cutting peptide chain stepwise, hypomolecular digested cytochrome c was obtained. The inhibiting effect of this substance against in vitro and in vivo anaphylactic reaction was tested in the guinea pig and the following results were obtained.
1. The sensitizing activity was found to be existing in cytochrome c digested within for 2 hrs, but it decreased remarkably in the material digested for over 3 hrs and by 15 hrs digestion it was lost completely.
2. The desensitzing activity of digested cytochrome c was tested in the guinea pig sensitized with intact cytochrome c. The desensitizing activity was demonstrated in the cases treated with cytochrome c digested for 3 hrs and 9 hrs. In the guinea pig desensitized with digested cytochrome c anaphylactic shock and Schultz-Dale reaction by intact cytochrome c were clearly inhibited.
3. Indicating I¹³¹labeled growth hormone and insulin, cytochrome c digested for 3 hrs and 9 hrs were fractionated with column chromatography using Sephadex 'G-50. Consequently, the main fraction was consisting of undigested cytochrome c, having molecular weight of over 25, 000 and halfway- digested cytochrome c, having molecular weight of about 6, 000. The inhibiting action of this fraction against anaphylaxis with undigested cytochrome c was also demonstrated.