Abstract
Studies were performed on the effect of fat metabolism on a relapse of Vitamin B2 deficiency disease, and effectiveness of various kinds of Vitamin B2 preparation against such relapsed conditions.
Results were obtained as follows :
1) Symptoms of Vitamin B2 deficiency could be detected much earlier in the group of rat with relapsed Vitamin B2 deficiency disease as compared with those of incipient B2 deficiency disease.
Also gain in weight and recovery from symptom of B2 deficiency after administrating Vitamin B2 preparation were observed to be delayed in former group.
2) Total amount of Vitamin B2 in whole blood showed 4.5γ/dl (ester type, 3.5γg/dl) on the average on the first day of study in the convalescent stage.
However, the average level of Vitamin B2 increased to 6.7γ/dl (ester type 5.7γ/dl) 25 days later, which revealed less lower than the average of 7.2γ/dl (ester type 6.4γ/dl) measured in the convalescent phase of the first experiment reported before. In case of dead specimen, total amount Vitamin B2 contained in whole blood showed average of 3.5γ/dl (ester type 2.5γ/dl) .
3) After diet for relapse of Vitamin B2 deficiency food was given, the increasing rate of lipid in serum was much larger as compared with that of the first experiment which reported before.
Especially, the group of rats fed by food which contained high amount of fat showed extremely higher level in serum lipid than those of groups which fed by medium and low amount of fat contained.
Significant difference of variation in amount of lipid in sera among these three groups, however, was not observed.
4) When Vitamin B2 preparation was given to the rats with relapsed Vitamin B2 deficiency disease, the delayed recovery from clinical aspects and improvement of fat metabolism were seen. Some of the rats died without showing any reactions even Free B2 daily or B2-Butylate every four days.
As the conclusion, daily dispense of B2-Butylate for B2 dificiency disease is believed to be an excellent prescription.
5) When symptom of B2 deficiency disease relapses, it tends to get worse very quickly and a high degree of B2 deficiency can be observed.
Thus, if the B2 preparation is not given at a proper time, it will be longer be effective and complete recovery could not be expected when B2 preparation is dispensed after tissues have already changed.
