Abstract
Rabbits premedicated daily for 3 weeks by the combination of steroid hormone and insulin were inoculated with bile or liver homogenate obtained from the patients with type A hepatitis and non-toxic fluminant hepatitis.
Premedicated rabbits were divided into 4 groups according to inoculation samples.
I group ; bile from type A hepatitis patients.
II groups : bile from fluminant hepatitis.
III group : liver homogenate from fluminant hepatitis.
VI group : homogenate from rabbit liver, induced tissue lesions by inoculation in above group. Rabbits were sacricefied 4, 10, 14 and 22 days after inoculation, respectively.
Histological findings were characterized by multifocal cell necrosis, particularly in the para-lobular areas and infiltration of polymorphonuclear leucocytes, with part of the mononuclear cells surrounding the sites of cell necrosis and focally in the potal areas. These findings were observed in five cases in eleven rabbits, in group I, 4/5 in group II, 1/4 in group III, 5/13 in group VI.
No cytoplasmic inclusion bodies were detected in hepatocytes by the Orcein staining method. No virus-like particles were detected in cytoplarm or nucleus of hepatocytes by electronmicroscopic analysis.
