Abstract
In this study, we developed a new method for the determination of α-amylase activity with maltopentaose as a substrate. Alpha-amylase activity is calculated numerically from the rate of glucose formation coupled with α-glucosidase.
This method proved to be capable of rapid and precise results, and its results correlated satisfactorily with the starch-dye method for serum samples (r=0.941) .
Apparent Km value of maltopentaose for salivary and pancreatic type amylase are 5.26 × 10-3 mol/l, and 2.89 × 10-3 mol/l, respectively.
