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Volume 41 (1981)
- Issue 6 Pages 619-
- Issue 5 Pages 481-
- Issue 4 Pages 353-
- Issue 3 Pages 227-
- Issue 2 Pages 117-
- Issue 1 Pages 1-
Volume 41, Issue 3
Displaying 1-14 of 14 articles from this issue
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1981 Volume 41 Issue 3 Pages 227-232
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSDownload PDF (1985K) -
Hiroshi HOJYO1981 Volume 41 Issue 3 Pages 233-240
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSIn the present study, healthy Albino-Rabbits (males), weighing about 2 Kg were used as models. Quadriceps femoris muscle obtained from 7 normal rabbits, and 11 posterior members were studied : Cross-sections and oblique sections were observed under S E M and the results were as follows :
(1) Diameter of muscle fiber was 10.1 to 32.0 μm and the cross-section was roundish polygonal. Muscle fiber bundle was formed of 30 to 50 muscle fibers and was surrounded by perimysium.
(2) It were determined that the most convex face of the regular risings in myofibril expresses M-line and, the concave face, Z-zone.
(3) Muscle fiber could be separated into 3 types : Roundish small-diametered muscle fiber, angularly polygonal large-diametered muscle fiber and polygonal roundish muscle fiber with intermediate diameter.
(4) As a result of observation of rectus femoris muscle and each vastus at the origin, there were no noticeable differences in forms and arrangement of muscle fibers in the vicinity of muscular insertion and ventral center,View full abstractDownload PDF (6806K) -
SCANNING ELECTRON MICROSCOPIC OBSERVATION OF SKELETAL MUSCLE DUE TO INTRAMUSCULAR INJECTION OF DRUGSHiroshi HOJYO1981 Volume 41 Issue 3 Pages 241-254
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSIn the present study, healthy Albino-Rabbits (males), weighing about 2 Kg were used as models. In single-injection group, 25 %-Sulpyrine, Restamin and Keflodin were injected into the thigh of the left posterior member and the right thigh was injected a 10 mm depth with physiological saline, as control. Repeated-injection group (10 times injections each days) was treated with 25 % Sulpyrine and control group was treated with physiological saline. The injected muscle area was observed under scanning electron microscope (S E M) 3, 7 and 28 days after treatment in single-injection, and 28 days. 3 and 6 months after treatment in repeated-injection group. Some muscle specimens were observed under optical microscope. With 42 rabbits, or 84 posterior members, the influence after intramuscular injection of drugs was studied by observation under S E M and optical microscope.
The following results were obtained :
(1) Influences after single injection, diastasis of intermuscle fiber bundles and intermuscle fibers, decollement of perimysium, coarseness of myofibril and necrosis-like struc-tural destruction were noted.
(2) In the control group of (1), diastasis of intermuscle fiber bundles and inter-muscle fibers were noted and mild coarseness of myofibril was seen in just a few specimens.
(3) After single injection, the influence of Sulpyrine on myofibril was comparable to that of Restamin, while that of Keflodin was mild and almost no influence on myofibril was seen in control group.
(4) After repeated injection, various changes such as very extensive necrosis-like structural destruction, alveolar structure, extreme coarseness, union of myofibril, etc. were seen in whole view. In control group, extreme coarseness was noted in general, and alveolar structure, tendency of necrosis-like structural destruction and abnormalities of myofibril in form and in arrangement were seen in a few specimens.
(5) In observation time, the muscular changes were found considerably restored after 28 days as compared with situation seen 3 and 7 days after single intramuscular in-jection and after 6 months as compared with situation seen 28 days and 3 months after repeated intramuscular injection. However, there remained some changes which were not expected to occur with time.
(6) As a result of this study, we determined that intramuscular injection of drugs will invade myofibril and induce irreversible degeneration.View full abstractDownload PDF (16577K) -
(ESPECIALLY THE LHRH-CONTAINING NEURONS)Naosuke MAEDA1981 Volume 41 Issue 3 Pages 255-264
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThe origin of nerve fibers, especially LHRH neurons, which project into the organum vasculosum laminae terminalis (OVLT) was investigated in the rat by electron microscopy, consequent to electrolysis of the various areas of the hypothalamus and immunocytochemistry using anti-LHRH serum. Two days after electrolysis of the medial preoptic area, a large number of degenerated nerve fibers and terminals was observed in the OVLT. When the posterior half of the anterior hypothalamic nucleus and the posterior third of the suprach-iasmatic nucleus were electrolyzed, no degenerated nerve fibers were detected in the OVLT. In a partial electrolysis of the medial preoptic area, degenerating profiles were detected in the LHRH immunoreactive neurons.
The presence of a preoptico-terminal pathway was demonstrated and this pathway was found to contain the LHRH-containing neurons (preoptico-terminal LHRH pathway) .
Electronmicroscopically, it was suggested that the immunoreactivity of LHRH is acquired only in the early stage of degeneration.View full abstractDownload PDF (9853K) -
Ryuichi UZAWA, Yasushi TAKAGI, Kunihide GOMI, Toru ISHII1981 Volume 41 Issue 3 Pages 265-269
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSIn this study, we developed a new method for the determination of α-amylase activity with maltopentaose as a substrate. Alpha-amylase activity is calculated numerically from the rate of glucose formation coupled with α-glucosidase.
This method proved to be capable of rapid and precise results, and its results correlated satisfactorily with the starch-dye method for serum samples (r=0.941) .
Apparent Km value of maltopentaose for salivary and pancreatic type amylase are 5.26 × 10-3 mol/l, and 2.89 × 10-3 mol/l, respectively.View full abstractDownload PDF (478K) -
—THERMAL SENSATION OF PATIENTS FOR THERMAL ENVIRONMENT IN HOSPITAL WARDSJunin MATSUI1981 Volume 41 Issue 3 Pages 271-284
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThroughout a complete year, continued measurements of temperatures and humidities in our hospital wards for patients with internal diseases were done and simultaneously the questionaire to the patients concerning their general thermal sensations, local feelings of warm and cold, humidity sensations, feeling of air circulation, feeleing of radiation heat, general thermal comfort, their clothing and bedding conditions was issued and their answers, were collected. Their charts were also investigated simultaneously, and the summarized results were as follows.
1. The optimal temperature in the wards in autumn was 22-23°C: in winter, 20-21°C: in spring, 21-22°C: and in summer, 24-25°C.
2. Young people reacted more sensitively to the room temperatures, that is, They feel cooler than others in cool rooms and warmer in warm rooms.
3. The diseases were classified into 5 groups by the tendency of feeling temperatures; namely, Type 1: always feeling it warmer than it really was ; Type 2 : feeling warmer when it was warm and colder when it was cold ; Type 3 : feeling cooler when it was warm and warmer when it was cool ; Type 4 : feeling cooler always than it really was and Type 5 : showing no particular inclination. It was desired that optimal thermal conditions would established according to type of diseases
4. “Too cool environment” is apt to be created by summertime cooling and “too warm environment” by wintertime heating is often created. The complaints of localized warm or cold feelings were most widely ranged in autumn, which suggested that timing for start of cooling or warming, periods for that practice and methods, etc. should be more carefully considered.
5. With regards to the feeling of temperature vs. humidity, “too dry” sensation in wintertime was pointed out. The difference in feeling of humidity depending on season was observed to be in the range of 20-39% relative humidity.
6. The patients adjusted their body temperatures by changes in clothings and beddings individually in order to meet the changes of thermal conditions in the wards, and within that range, the hospitals at present having air-conditioning facilites are in satisfactory conditions with regards to the thermal environment creation.
7. However, problems still remain to be solved in the future for patients with severe conditions or for infants who are unable to adjust their body temperatures by themselves.View full abstractDownload PDF (1295K) -
Masaaki CHIGA1981 Volume 41 Issue 3 Pages 285-293
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThis study, carried out using mongrel dogs in which we induced hypotension by S. N. P. and T. M. P., represents our efforts to measure the carbohydrate metabolism, acidbase balance and hemodynamics during a hypotensive situation. During hypotension, mean arterial pressure ranged from 50 to 60 percent of pre-examined levels.
The results are as follows:
1) T. P. R. decreased significantly during hypotension in S. N. P. group. In T. M. P., both T. P. R. and C. O. decreased significantly.
2) A significant increase of blood glucose was found in S. N. P. group, but not in T. M. P. group.
3) Lactate, L/P ratio and excess lactate increased significantly in S. N. P. group, but not in T.M. P. group.
4) In both groups, PvO2 tended to increase, but the decrease of A-vCDO2 and VO2 was not seen.
From the above results, it was suggested that the hypotensive action of S. N. P. was mostly due to the decrease of T. P. R., and that of T. M. P. was due to the decrease of both T. P. R. and C. O., furthermore, S. N. P. tended to lead tissue to hypoxia more markedly than T. M. P.. It can be concluded that induced hypotension by S. N. P. tends to cause metabolic acidosis due to the anaerobic metabolism brought by the deterioration of peripheral circulation or cyanide poisoning. In adaition, on T. M. P, has a tendency to maintain peripheral circulation comparatively well.View full abstractDownload PDF (969K) -
CHARACTERISTICS AND EFFECTS OF INHIBITORS ON MITOCHONDRIAL MAO IN RABBIT LIVERTohru HASHIMOTO1981 Volume 41 Issue 3 Pages 295-302
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSStudies on multiplicity of mitochondria MAO in rabbit and rat liver were carried out, in vitro and in vivo, by investigating differences in the substrate specificity and inhibitor sensitivity to various MAO inhibitors, such as harmine, harmaline and clorgyline (type A MAO inhibitors) and deprenyl and pargyline (type B MAO inhibitors) . The results obtained with these two preparations were compared. The rabbit liver MAO showed the greatest rate of oxidation of β-phenylethylamine (PEA), a specific substrate for type B MAO, but showed the weakest rate of oxidation of 5-hydroxytryptemine (5-HT), a specific substrate for type A MAO. On the other hand, the rat liver MAO greatly oxidized tyramine, a substrate for both types of MAO, and it oxidized both 5-HT and PEA at a relatively smaller rate. The type A MAO inhibitors, clorgyline and harmine, caused a weak inhibition for rabbit liver MAO activity with PEA and benzylamine as substrates, but clorgyline strongly inhibited activity towards 5-HT, whereas deprenyl slightly inhibited the activity. In rat liver, type A MAO inhibitors strongly inhibited activity towards 5-HT, but it was these found to be weak for activities towards PEA and benzylamine. Inhibition curves of activity towards tyramine, by these type A MAO inhibitors, were found to be all double-sigmoidal. With deprenyl as inhibitor, almost similar results were obtained, but benzylamine oxidizing activity was the most sensitive to this inhibitor. After pre-treatment with harmaline, inhibitory action of pargyline to oxidation of 5-HT was remarkably reduced, but with other substrates, this pre-treatment did not show any remarkable protection against inhibitory effects of paryyline. This non-protective effect of the pre-treatment was also observed for rabbit liver MAO with these substrates.
These results suggest that mitochondria of rabbit and rat liver would contain both types of MAO. but the former predominantly contains type B MAO.View full abstractDownload PDF (942K) -
MULTIPLICITY OF MITOCHONDRIAL MONOAMINE OXIDASE IN RABBIT LIVER—DIFFERENCES IN THE AFFINITY FOR OXYGENTamio KUSHIHASHI1981 Volume 41 Issue 3 Pages 303-310
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSIn order to know the enzymic character of mitochondrial monoamine oxidase (MAO) in rabbit liver, substrate specificities, effects of oxygen concentration and several inhibitors on MAO were studied. MAO in rabbit liver could oxidize tyramine most strongly, coming β-phenylethylamine (PEA) in the next, while the oxidation of 5-hydroxytryptamine (5-HT) was weakest among the substrates examined. Deprenyl and pargyline, specific inhibitors for type B MAO, strongly inhibited MAO activities with all substrates examined but clorgyline, a specific inhibitor for tyre A MAO, inhibited MAO in rabbit liver very little with any substrate used. From differences of affinity for oxygen, the mitochondrial MAO in rabbit liver could be divided into three groups unrelated to type A and B MAO. In the case of benzylamine, amylamine, 5-HT and butylamine as substrate, the affinity of MAO for oxygen was found to be high, while the affinity was very low with PEA and hexylamine as substrate. The affinity for oxygen obtained with tyramine and tryptamine as substrate was situated between those obtained with benzylamine and PEA. The order of affinity of MAO for oxygen was not relate to that of substrate specificity. The affinity for oxygen of MAO in rabbit liver was not changed by the addition of MAO inhibitors, such as clorgyline, deprenyl and pargyline. From these results, in regard to substrate specificities to MAO inhibitors, clorgyline, deprenyl and pargyline, it was suggested that the mitochondrial MAO in rabbit liver mainly consisted of type B MAO, but in regard to the affinity of MAO for oxygen, this enzyme could be composed three different forms.View full abstractDownload PDF (884K) -
Michio SATO1981 Volume 41 Issue 3 Pages 311-319
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThe duration of negative after potential (NAP) of toad striated muscle (sartorius and abdominis) was prolonged and slightly potentiated in Ca free medium. In spite of reduction of spike potential in these medium, the hight of contraction was not changed significantly.
This effect was further enhanced by adding 1.3 mM EDTA to Ca free medium.
1.2 mM CdCl2 also prolonged the duration of NAP. This prolongation of NAP by CdCl2 was further increased in Ca free medium.
TEA-Ringer greatly prolonged NAP. This effect was further enhanced in Ca free medium.
In glycerol treated muscle, NAP itself and Ca depletion, effect on NAP disappeared.
These data indicate that the increased NAP duration in Ca free medium might be attributed to the prolonged Na influx into T-tubule under Ca depletion, since prolongation of NAP in Ca free medium was independent of that caused by Cd and TEA.View full abstractDownload PDF (838K) -
Hideo TAKATSUKI1981 Volume 41 Issue 3 Pages 321-336
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSTo clarify the constitutional background of chronic diseases, 338 adult male Japanese in-patients admitted to the Dept, of Internal Medicine, Sasa Hospital, Tokyo were somatotyped using Sheldon's method, based on the observation of standard photographs and the height/weight-ratio index.
The subjects were classified into five clinical groups, i. e., hypertensive, chronic digestive, sugar metabolic (diabetic), chronic hepatic and other non-chronic diseases groups. The mean somatotypes for these groups were 4.46-4.53-2.20 (endomorphic mesomorphy), 3.4-4.5-3.0 (mesomorphy), 4.3-4.6-2.3 (endomorphic mesomorphy), 3.8-4.3-2.8 (mild endomorphic mesomorphy) and 3.3-4.5-3.3 (mesomorphy), respectively. Statistical analysis of three somatotypic components showed some significant differences between groups in somatotype distribution.
The mesomorphic (second) component was relatively dominant in every group, as in the normal Japanese male population. The endomorphic (first) component was high in the hypertensive and the sugar metabolic groups, and moderately found in the chronic hepatic group. The ectomorphic (third) component showed a lesser degree of development in the groups of chronic diseases than in the group of non-chronic ones.
From the results, it is suggested that some chronic diseases are closely related to constitutional factors, which are manifested as somatotypological characteristics.View full abstractDownload PDF (4426K) -
Tadasuke NISHIMURA1981 Volume 41 Issue 3 Pages 337-344
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSPharmacological studies of experimental hyperlipemia and atherosclerosis induced by 1.0% cholesterol diet (Ch-diet), 20% saccharose diet (S-diet) and 0.5 % cholesterol-20 % saccharose combined diet (CS-diet) were investigated by the measurement of serum lipid levels and morpholigical change of aortic arch using scanning electron microscope. Serum total cholesterol (TC) level began to rise 2 weeks after fed on Ch-and CS-diet and reached the peak 8 to 10 weeks later, which lowered in both Ch-and CS-diet groups 12 weeks later. Serum triglyceride (TG) level increased by fed on CS-diet only. An increase of serum β-lipoprotein was similar to that of TC in both Ch-and CS-diet groups. An increase of TG induced by CS-diet was greater than Ch-diet group.
Change of serum lipid levels by S-diet showed that TC and β-lipoprotein slightly increased. Luminal surface structure of aortic arch using scanning electron microscope showed the swelling of nucleus by fed on Ch-diet for 8 weeks, and an increase of swollen nucleus and appearance of edematous structure for 16 weeks. Change of luminal surface induced by fed on CS-diet for 12 weeks was similar to that of fed on Ch-diet for 16 weeks, and showed edematous structure of all over the luminal surface after for 16 weeks. Change of luminal surface fed on S-diet for 12 weeks showed edematous structure. Experimental atherosclerosis in rabbits were accelerated by addition of saccharose in Ch-diet.View full abstractDownload PDF (8632K) -
Ryosaku ITO, Teizo AJIRI, Hiroshi NAKANISHI, Yuji MATSUMOTO, Yoichi KA ...1981 Volume 41 Issue 3 Pages 345-346
Published: June 28, 1981
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThe flexor capri radialis profundus was found on the both sides of the forearm of a Japanese male corpse aged 76 years, and was compared with the data in past literature. It was delermined that the anomalous muscles in our subject were formed by division of the flexor pollicis longus.View full abstractDownload PDF (239K) -
A CASE REPORT OF BENIGN CYSTIC TERATOMA IN THE THORAX COMPLICATED WITH INTERCOSTAL-PULMONARY FISTULAFumio MATSUMOTO, Mitsugu HASEGAWA, Toyosuke INOUE, Takashi KATAGIRI, K ...1981 Volume 41 Issue 3 Pages 347-351
Published: June 28, 1981
Released on J-STAGE: November 19, 2010
JOURNAL FREE ACCESSIn this study, a rare case of benign cystic teratoma in the thorax complicated with the intercostalpulmonary fistula, is reported. The patient was a 41-year-old female who suffered from pleuritis when she was aged 28. She had no physical complaints, but when she had an X-ray examination in the health check-up, a tumorous silhouette was discovered on the left margin of the heart. Only a systolic murmur was a predominant physica finding. Affer several examinations, she was diagnosed as having a benign cystic teratoma in the thorax complicated with an intercostal-pulmonary fistula. Being afraid of some complications in the future, the operation was performed with good result.View full abstractDownload PDF (6270K)
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