Abstract
Pharmacological studies of experimental hyperlipemia and atherosclerosis induced by 1.0% cholesterol diet (Ch-diet), 20% saccharose diet (S-diet) and 0.5 % cholesterol-20 % saccharose combined diet (CS-diet) were investigated by the measurement of serum lipid levels and morpholigical change of aortic arch using scanning electron microscope. Serum total cholesterol (TC) level began to rise 2 weeks after fed on Ch-and CS-diet and reached the peak 8 to 10 weeks later, which lowered in both Ch-and CS-diet groups 12 weeks later. Serum triglyceride (TG) level increased by fed on CS-diet only. An increase of serum β-lipoprotein was similar to that of TC in both Ch-and CS-diet groups. An increase of TG induced by CS-diet was greater than Ch-diet group.
Change of serum lipid levels by S-diet showed that TC and β-lipoprotein slightly increased. Luminal surface structure of aortic arch using scanning electron microscope showed the swelling of nucleus by fed on Ch-diet for 8 weeks, and an increase of swollen nucleus and appearance of edematous structure for 16 weeks. Change of luminal surface induced by fed on CS-diet for 12 weeks was similar to that of fed on Ch-diet for 16 weeks, and showed edematous structure of all over the luminal surface after for 16 weeks. Change of luminal surface fed on S-diet for 12 weeks showed edematous structure. Experimental atherosclerosis in rabbits were accelerated by addition of saccharose in Ch-diet.
