A CASE OF ABNORMAL HEMOGLOBIN IN ERYTHROLEUKEMIA

Abstract

By supravital staining of blood from an erythroleukemic, we observed erythrocytes which were golf ball-like in appearance, suggesting abnormal hemoglobin presence. In hemoglobin electrophoresis by starch gel, the abnormal extraband was mobilized faster than the Hb A. Then, we purified this abnormal extra-band by anion-exchange column chromatography, DEAE-Sepharose CL-6B. Purified, this abnormal fraction, which was treated with PCMB (p-chloromercuribenzoic acid), was then electrophorized, and only beta-chain and non-dissociated he-moglobin were detected: alpha-chain was not. These results suggested this abnormal hemoglobin was Hb H.