Abstract
Adjuvant activity of DNP-Ficoll was found in culture system using SRBC-primed spleen cells (or B cells separated from the activated spleen cells) . The spleen cell suspensions were cultured for 48 hr at 37°C after addition of DNP-Ficoll, and then, the number of antiSRBC PFC was determined by the method of Cunningham and Szenberg. When the spleens excised from ICR mice 4 and 6 days after immunization with SRBC antigen were used, increase in anti-SRBC (IgM) PFC number was observed in the cultured cells in the presence of DNP-Ficoll (0.02μg/ml) . The values from the treated and control cultures were 351±29.0 and 240±23.8/106B cells, respectively. This adjuvant material did not affect production of soluble factors from T cells or macrophages. Only when B cell and T cell co-existed with macrophage in the culture, we have obtained increased number of PFC nearly equal to that in ICR mouse spleen cells.
