Abstract
To investigate the regulation of rat pancreatic secretion, we examined upper intestinal perfusion in conscious rats during diversion of bile-pancreatic juice (BPJ) . Diversion of BPJ from the upper intestine greatly increased pancreatic secretion associated with the elevation of plasma CCK. From this result, we hypothesized that a trypsin sensitive CCK releasing factor in the upper intestinal lumen, and that diversion of BPJ retains its activity from protease digestion. We perfused the upper intestine with phosphate buffer saline (PBS) and observed pancreatic volume, protein, bicarbonate output, and plasma CCK. Washing the upper intestine with PBS decreased all pancreatic secretion and plasma CCK. From this result, we believe that an intraluminal CCK releasing factor regulates CCK release, and our putative CCK releasing factor is different from the monitor peptide that exists in pancreatic juice.
