Abstract
Well-defined basement membrane laminin (LN) was seen in 93 progressive colorectal carcinomas stained by immunohistological methods (LN and VB-LN double stain) using 10% formalin fixed specimens to clarify relations between LN positivity of the primary tumors and their liver metastasis. Among these 93 colorectal carcinoma cases, 22 were affected by simultaneous liver metastasis (H (+) group), and LN staining was positive in 48 (51.6%) . Positive LN staining was found in 17 liver metastasis cases (77.3%) of H (+), and 31 cases (43.7%) of H (-), a statistically significant difference (P<0.01) . There was no correlation between LN staining and macroscopic appearance, histologic type, depth of infiltration, or v, ly, and n factors. In LN staining of primary lesions, positive rates of intravenous cancer nests (vLN) were 54.5% of H (+) and 8.2% of H (-), which were significantly different (P<0.01) . There was correlation between LN positivity of primary lesion and vLN. Liver metastasis was recognized in more than 80% of the patients that were proved v2-3, and simultaneously LN staining or vLN positive. Also, among ssv (+) cases, more patients with liver metastasis were positive for both LN and vLN than were patients without metastasis (P<0.05) . These data indicate that LN and vLN positive staining of primary lesions might appear in a group at high risk for liver metastasis, and investigations of simultaneous LN staining and venous invasion could be useful for predicting liver metastasis. VB-LN double staining, by which it is possible to examine both LN staining and v studies from the same specimen, could be a viable method.
