Abstract
Object : To determine whether the mechanism of absorption of macromolecules in allergic intestines is IgE dependent.
Materials and Methods : First, rats sensitized to ovalbumin were given ovalbumin orally. Then, after 1 hour β-lactoglobulin was instilled orally. We measured the concentrations of β-lactoglobulin with enzyme-linked immunosorbent assay for 4 hours.
β-Lactoglobulin was detected in the serum of the rats after 30-minutes, and the maximal peak concentrations of β-lactoglobulin were at 60, 120, and 240 minutes. Second, after the sensitized rats received ovalbumin orally, they were divided into five groups (1, 2, 3, 4 and 5) that received β-lactoglobulin 1, 3, 6, 12 and 24 hours, respectively, after receiving ovalbumin. We then measured 2-hour serums concentrations in each group because it has been reported that 2-hour values in the absorption of macromolecules are maximal in intestinal inflammation. The concentrations in each group were compared.
Result : We could detect β-lactoglobulin in the rats of groups 1, 3 and 5. However, we could not detect in groups 2 and 4. Therefore, the absorption of macromolecules showed different patterns.
Conclusion : Our findings suspect that the mechanism of absorption in the allergic intestine is not IgE dependent immediate response but late response, post late response and by these the absorptions of macromolecules are affected.
