Abstract
This study examined whether calcium administration before ischemia as preconditioning reduced ischemic reperfusion injury.‹Methods›Isolated rat hearts were perf used for 1 minute with five kinds of Krebs-Henseleit bicarbonate buffer each with a different Ca concentration Experimental group : Groupl (control), Groups 2, 3, 4, 5 : Ca = 2.5, 3.0, 3.5, 4.0, 5.0mM/L respectively f 30 minutes before global ischemia at the normothermia (37.5°C) .
Cardiac function (Heart rate : HR, left ventricular developed pressure : LVDP, Left ventricular pressure dp / dt : LV dp / dt, coronary flow: CF) were measured during 30 minutes after reperfusion and compared with baseline. The calcium ion concentration was measured in group 3.‹Results›Groups 2, 3, 4, 5 showed a better recovery than the control group in LVDP, LVdp/ dt and CF after reperfusion. In addition, the cytosolic calcium concentration did not increase during ischemia/reperfusion compared with the control group.‹Conclusions›These results suggest that preconditioning with calcium produces tolerance to the subsequent ischemia/reperfusion and reduces reperfusion injury.
