Abstract
In 1997, we examined the DNA-synthesis stage cells of the cleft palate produced by Vitamin A. We have made a comparative study of 3 groups since then. Group VE: On days 7 and 8 of pregnancy, Vitamin E 12.5mg/100g weight was administered on days 9 to 11; Vitamin A 10, 000I.U./100g weight was done for 30 mother rats. Group VA: Vitamin A alone administered for 30 mother rats. Group C: 25 mother rats were the control. On the 21st day of pregnancy, the mother rats were sacrificed after the administration of BrdU to observe the 65 fetuses as samples of DNA-synthesis stage cells under a microscope. The incidence rate of cleft palate in this examination was 47.0% in the VE group, 73.5% in the VA group and 0% in the C group. The effect of Vitamin E on incidence control of the cleft palate produced by Vitamin A was 64.0%. Subsequently, the average of DNA-synthesis stage cell positive rate in the VE group was 16.0‰, 85.2‰ in the VA group and 7.7‰ in the C group. The effect of Vitamin E is anti-oxidant and is thought to restrain the incidence of cleft palate by preventing the production of the oxide of Vitamin A (=retinol acid) . In conclusion, we hypothesize that retinol acid disturbs the increase of cells although we could not determine the mechanism in this study.
