Abstract
Oxidative stress may contribute to many pathophysiologic changes that occur after subarachnoid hemorrhage. 8-hydroxy-2'-deoxyguanosine (8-OHdG), proposed to be a biomarker for DNA damage by oxidative stress, and 8-iso-prostaglandinF2α (8-iPGF2α) are prostaglandin isomers that are formed nonenzymatically by free radical-induced oxidation of arachidonic acid. 8-iPGF2αis also proposed to be an oxidative stress marker of cell membrane damage. Measurement of 8-OHdG and 8-iPGF2αmay offer a sensitive, specific, and noninvasive method for measuring oxidant stress in clinical practice. In this study, simultaneous measurements of both stress markers in subarachnoid hemorrhages were carried out. Urine was collected every day over two weeks from 7 patients with subarachnoid hemorrhage and analyzed with an ELISA Kit. There was a significant correlation between the two markers in both the rate of production (p<0.001, r=0.487) and urinary concentration (p<0.001, r=0.564) . We also observed the time course of the two markers. The levels of 8-OHdG and 8-iPGF2αshowed a similar tendency: a marked elevation in the 2nd or the 3rd, 6 days relative to the other days. These data suggest that oxygen radicals produced by subarachnoid hemorrhage would cause similar damage to DNA and the cell membrane, The sequential change of 8-iPGF2αindicates the occurrence of cerebral ischemia due to vasospasm following subarachnoid hemorrhage.
