Abstract
Chemotherapy against hormone refractory prostate carcinoma has been investigated. Although occasional responses have been seen, prostate cancer still which shows low responses to chemotherapy. One of the factors which makes cancer cells acquire resistance is heat shock protein (HSP) while cycloheximide (CHX) is known as a substance which inhibits HSP function. Thus, we hypothesized that administration of CHX prior to heat treatment could raise effectiveness an against anticancer reagent. On MTT assay in chemotherapy revealed that heat treatment inhibited efficacy of cisplatinum (CDDP) and THP-adriamycin ( THP) . In addition elevated HSP expression was detected with heat treatment by western blot analysis. From the results, it could be said that HSP inhibited efficacy of CDDP and THP. Subsequently, CHX was administrated prior to the anticancer reagent. The HSP expression was inhibited and CHX made warm the chemothermotherapy more effective. We conclude that CHX heightens the effectiveness of chemotherapy.
