2002 Volume 62 Issue 4 Pages 237-247
Previous studies have implicated advanced glycation end products (AGEs) in the pathogenesis of diabetes and its complications. However, metabolic pathways and the distribution of AGEs in various organs and tissues are still not clear. Pratically, only one report, a case of distribution of carboxy-methyl-lysine (CML) in a non-diabetic human, has been reported. Using two mouse anti-human antibodies for AGES, 6D12 and H12, 6D12 recognize Carboxymethl-lysine (CML) and H12 recognized Pyrraline. With a simple stain method, we examined the immunohistological distribution and localization of AGEs in various organs and tissues obtained form 50 diabetic autopsy cases (35 men and 15 women, 42 to 93 years of age, mean 72.1 years old) and 10 non-diabetic autopsy cases (6 men and 4 women, 0 to 77 years of age, mean 50.2 years old) . We valued the results as positive rates. Some organs and tissues showed high positive rates in both CML Pyrraline. In the non-diabetic autopsy cases, mostly pancreatic and renal tissues showed low positive rates of both CML and Pyrraline, but high positive rates in the diabetic autopsy cases. It was an interesting that, only in the pancreatic and renal tissues of the non-diabetic cases, the distribution of AGES had been inhibited. There fore, we discuss the existence of some functions that inhibit distribution of AGEs in the pancreas and the kidney in the non-diabetic human, and unknown metabolic pathways or receptors of AGES.
