Abstract
Important pathologic findings of the peritoneal membrane in long term CAPD patients reveal thickening of the peritoneal membrane and on increase of vessels. These changes lead to the increase in permeability of the vessel and the development of peritoneal membrane ultra filtration failure. Excessive vessel formation in the peritoneal membrane and the resultant peritoneal failure may be caused by the change in balance between the ELR motif (+) CXC chemokines and ELR motif (-) CXC chemokines. ELR motif (+) CXC chemokines (GRO-α IL-8) and ELR motif (-) CXC chemokines (MIG IP-10) from cultured HPFbs by inflammatory cytokine (TNF-α IFN-γ) stimulation were assayed by ELISA and analyzed. INF-y suppressed production of ELR motif (+) CXC chemokines from HPFbs and promoted production of ELR motif (-) CXC chemokines from HPFbs in a time and dose dependent manner. TNF-α promoted production of ELR motif (+) CXC chemokines from HPFbs in a time and dose dependent manner. The combination of INF-γ and TNF-a had a synergic effect on the production of ELR motif (-) CXC chemokines from HPFbs in a time and TNF-α or IFN-γ dose dependent manner. These results 1) : suggest that IFN-γ and TNF-α have a synergic effect on the production of angiogenic or angiostatic CXC chemokines from HPFbs, and 2) : further supported the concept that with inflammation of the peritoneal cavity and a change in the biological ELR balance of CXC chemokines, the peritoneal membrane may be affected by angiogenic or angiostatic CXC chemokines.
