抄録
Recently, the construction of 3-dimensional internal structure images of biological samples has been used for vital observation. To construct the image, the sample is sliced into many sections with a machine such as a microtome. Then images of the sections are acquired by the system. By acquisitioning the images, a 3-dimensional internal structure image of the sample can be created. To realize the mentioned observation system, we proposed a new type of micro-slicer image processing system with a direct translation blade and automatic sample feeding mechanism. An image scanner (contact image sensor) was attached in the system to obtain 2-dimensional images from the sliced sample, not from the sliced section. By using an image scanner called a micro-slicer image processing system instead of a CCD camera, which is used in conventional systems, line lighting of the image scanner enables uniform illumination on the cutting planes of the samples. Additionally, the characteristics of an image scanner, such as shallow depth of field, an oblique illuminating system, and one-dimensional array of light-sensitive elements, enable us to display hollow portions of the sample. As a result, the calibration process of the 2-dimensional images obtained from the sample, which was needed in the conventional system, is no longer necessary. So we achieved the automation of constructing high-definition 3-dimensional images. Furthermore, the miniaturization of equipment was realized because the CCD camera and optical systems became unnecessary.
