For application of a cultured muscle to medical engineering, both to control C2C12 myoblast differentiation and to investigate a contraction mechanism of C2C12 myotube are important. The muscle can be derived from a cultured C2C12 myoblast in an incubator. It is important to evaluate a level of C2C12 differentiation quantitatively. In this study, we propose an efficient evaluation method by cellular membrane potential measurement instead of the conventional methods that take time and are higher cost.
