抄録
A system for real-time monitoring of cell population behavior is required to noninvasively evaluate cellular function and to assess the quality of the cell culture process. The purpose of this study has been to develop a real-time monitoring system using particle image velocimetry (PIV) for visualizing and measuring the behavior of individual cells in a cell population. Proliferation of mouse embryonic fibroblast cells (NIH3T3) plated at 0.005×104, 0.01×104, 0.02×104, 0.20×104, 0.50×104, 0.80×104 cells/cm2 was captured using the developed system over a 72-h period. Direction and rate of cellular migration in the cell population were analyzed. Results show that the migration rate of individual cells decreased as the plating density increased. Cells in a low-density culture exhibited a high migration ratio and the migrating direction deviated over time. On the other hand, cells in a high-density culture exhibited a low migration ratio and the migration direction did not deviate. In conclusion, PIV analysis indicates that the migration direction of individual cells depends on cell density.
