Abstract
Polarization-resolved second-harmonic-generation (SHG) microscopy is a powerful tool to visualize distribution of collagen fiber orientation in tissue with little invasion. However, long image acquisition time, resulting from mechanical rotation of a half-wave plate, makes this microscopy easy to suffer from motion artifact of a sample and hence has limited its use to in vivo application. In this paper, we constructed rapid, polarization-resolved SHG microscopy by combination of an electro-optics-modulator-based polarization modulation with improved data acquisition method. The constructed SHG microscopy enables us to visualize orientation mapping of dermal collagen fiber in rat skin in vivo without influence of motion artifact. This microscope will open the door for in vivo measurement of collagen fiber orientation in tissue.
