Experimental Verification of Spectroscopic Imaging Encoding Depth Information Based on Analyzing Cutaneous Blood Vessels by Spectroscopic Images and 3D Images

Abstract

We have contrasted spectroscopic images of blood vessels under a true skin surface of mouse with laser confocal scanning microscope 3D images of them, and analyzed these optical reflection densities of vessels. We have demonstrated that the difference between the optical reflection density of high absorption isosbestic spectrum and that of low depend on the depth of a vessel linearly. We have experimentally verified the possibility of spectroscopic vessel imaging encoding depth information.