光ピンセット内蔵型リアルタイム３次元顕微鏡システム

抄録

Optical tweezers is a major technique to manipulate cells. 3D observation method of cell manipulation is required for the purpose of visualizing rotations and 3D position. However, this method is especially difficult because optical path of both optical tweezers and 3D observation will interfere. We developed a system to resolve the difficulty with using a variable-focus lens (VFL). We continuously acquire slice images having modified focal position and reconstruct them to get 3D image. The VFL changes focal position rapidly and is set at a position not to interfere the optical tweezers. We succeeded to get slice images in 30 fps and to reconstruct into 3D images in the experiment.