一般演題（口演）
Molecular structure of matrices solubilized with HCl from decellularized liver and the production of albumin from hepatocytes cultured on the matrices
2014 年 52 巻 Supplement 号 p. O-194-O-195
詳細
2014 年 52 巻 Supplement 号 p. O-194-O-195
Solubilized liver-specific extracellular matrix (ECM) as a functional culture substratum was evaluated in this study. Briefly, the various types of solubilized ECMs were prepared from decellularized liver with HCl of various concentrations. The concentration of HCl affected the molecular structure of the solubilized ECMs. On the other hand, the level of albumin production from hepatocytes cultured on the solubilized ECMs films were higher than that of type I collagen film. However, the differences of cell adhesive tendency and albumin production of hepatocytes were could not be observed among various ECMs. Therefore, liver ECM composition is effective as a hepatocyte culture substratum.
