2014 Volume 52 Issue Supplement Pages O-198-O-199
We found a unique cell membrane perforation method which is applying photochemical oxidation on a limited area of cell membrane, with high survival rate. We recently developed a photosensitizer included nano needle array perforator for mass cell processing and the robotic processing system. The system had still some instability but in this study, we modified the whole system to improve the mechanical accurancy.The perforator is processed by nanoimprint, with a novel fine mold. The material is silicone polymer including photosensitizer Hematoporphyrin and iron oxide for magnetic connect to the processor. The dimensions of the needle are follows: diameter and hight 1.7μm, pitch 3.0μm. Excitation light is blue bandpass filtered 100W mercury lamp beam. With the system, we could inject membrane impermeable fluorescent dye AlexaFluor594 to rat PC12 cells and Human coronary artery endothelial cells. We will improve the system for various applications, e.g. transgenic process etc.
