Transactions of Japanese Society for Medical and Biological Engineering
Online ISSN : 1881-4379
Print ISSN : 1347-443X
ISSN-L : 1347-443X
Oral Session
A Novel Animal Model for Monitoring Bacterial Infection and Neutrophil Activity
Natasha ZulazizNaomi HimenoHiromi MiyazakiDaizoh SaitohAzran AzhimYuji Morimoto
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2014 Volume 52 Issue Supplement Pages O-285-O-286

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Abstract

Introduction: In order to simultaneously monitor neutrophil migration in vivo during inflammation, we developed a novel animal model for subcutaneous soft tissue infection using transgenic mouse bearing fluorescence-positive neutrophils and bioluminescent methicillin-resistant Staphylococcus aureus (MRSA).
Methods: Eight to nine-week-old male lys-EGFP C57BL/6 were anesthetized (50 mg/kg pentobarbital sodium, ip) and 1.0 ×107 CFU of bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected. Control group was injected with PBS while the treatment group was given Arbekacin (25 mg/kg) intravenously via tail vein. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed.
Results: Both groups displayed similar pattern with significant drop in MRSA bioluminescence and neutrophil fluorescence peaked on day 1. However, bacterial infection completely resolved in treatment group by day 6 with gradual decline in neutrophil fluorescence.
Conclusions: This animal model could be a competent method for assessment of subcutaneous soft tissue infections.

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© 2014 Japanese Society for Medical and Biological Engineering
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