2014 年 52 巻 Supplement 号 p. SY-50-SY-51
Mammalian cells utilize glucose as an essential fuel, taking it up in a stereoselective manner through GLUTs, whereby only D- and not L-, glucose is recognized. Cellular uptake of D-glucose through GLUTs can be monitored by 2-NBDG, a widely used D-glucose analogue emitting green fluorescence. For evaluating stereoselectivity of the uptake more precisely, we have developed 2-NBDLG, the first commercially available fluorescent L-glucose analogue (f-LG), as a negative control substrate for 2-NBDG. In the present talk, I introduce some critical features of 2-NBDLG when applied to mammalian cells as well as its unexpected turn.