Abstract
Reconstructed cardiac tissue equivalent does not have enough contractile function. In the present study, we assessed the effects of polyunsaturated fatty acid (PUFA), supplemented in medium, on fatty acid (FA) composition in cultured cardiomyocytes (CM). CM harvested from fetal rats were cultured for 14 days with linoleic (20 μM; LA group) or linolenic (10 μM; αLA group) acid supplementation in medium from the 4th culture day. At the end of culture period, FA composition was determined, and compared with that in CM cultured without PUFA (Cul group) and that in neonatal myocardium (Neo group). In LA group, linoleic, arachidonic, and docosatetraenoic acid contents were significantly higher than those in the Cul group (p<0.05, respectively), whereas arachidonic acid (AA) content was still much lower than that in Neo group (p<0.01). In αLA group, eicosapentaenoic acid content was significantly higher than that in Cul group (p<0.05), while docosahexaenoic acid (DHA) content was considerably lower than that in Neo group (p<0.05). The results suggest that linoleic or linolenic acid supplementation increases several PUFAs, except for AA and DHA, in cultured CM to their neonatal levels.
