Effect of Micropattern on Cell Orientation

Abstract

Development of the regenerative medicine has been remarkable. Cell is cultured in vitro, and returned in vivo by the medicine. Cell orientation enhances the one directional contraction of the tissue. Cell is known to make orientation when cultured on the micropattern. In this study, cells are cultured using various checks micropattern. Photolithography technique was used to make a micropattern. SU-8 of resist on the glass substrate was exposed using the mask and developed to manufacture micropattern. Aspect rate of pattern was prepared in 3 types. Vertical length of the quadrangle is fixed at 10 μm and the horizontal length was manufactured at 10 μm, 8 μm, and 5 μm. C2C12 cells were cultured on the checks pattern for 20 days. C2C12 cells were observed along the pattern, which differentiate into myotubes. Aspect rate of the manufactured checks micropattern affects orientation of C2C12.