電気刺激による筋芽細胞の移動への影響

抄録

We have been working on the development of bio-actuator using a titanium coil. However, micro titanium coil was not contracted. One of the problem is that cell did not adhere on micro titanium coil. Therefore, cell was collected near micro titanium coil using electrical stimulation. In this study, as a step before working with the micro-titanium coils, cells were subjected to electrical stimulation experiments using titanium wire. Titanium thin film of 100 nm thick was deposited on slide glass in rectangular shape using an electron beam deposition apparatus. Titanium wire was mounted in parallel to the titanium thin film. Electric pulse was used with the power supply voltage 10 V, duty ratio 50% and 1 Hz frequency. Cell was observed using phase contrast microscope. Evaluation method was used with time-lapse movie of 30 seconds per frame.