Recent advances in tissue engineering have led to the development of various artificial tissues, such as cell sheets and organoids, which are now being used in clinical settings. The progress made in the clinical application of cell sheets is especially remarkable. However, prior preparation of these sheets is a complex process and is a burden to patients. Cryopreservation of artificial tissues in clinical settings may help overcome these limitations. We have successfully cryopreserved tissues/organs, such as chondrocyte sheets, pancreatic islets, and fetal anlagens, via vitrification methods, which was developed through the cryopreservation of mammalian early embryos. Furthermore, we have demonstrated that the presence of carboxylated poly-L-lysine is effective for the stabilization of the vitrified state of the solution, increasing the integrity and survival of the vitrified tissues. This report focuses on the current progress and perspectives in artificial tissue cryopreservation, which will play an essential role in next-generation regenerative medicine.
