Development of the experimental setup to assess the Vitamin K₃'s fluorescent characteristics

Abstract

Recent study revealed that menaqunone-4 (MK-4) may be a preferred form among vitamin K (VK) homologues and it would play an important role in the body. There is also consistent evidence that the MK-4 is produced by conversion from dietary VK₁ and VK₂. VK₃ is an intermediate of the conversion reaction. If the serum VK₃ concentration can be detected, the relationship between some diseases and VK₃ would be clarified. The conventional method to detect VK₁ and VK₂ is the fluorescence detection method. Therefore, the relationship between VK₃ concentration and fluorescence amount has not ever been clarified. Consequently, the purpose of this study was to quantify the relationship between VK₃ concentration and fluorescence amount. We constructed the experimental system for the VK₃ fluorescence measurement. And we evaluated the relationship between the VK₃ concentration and the fluorescence amount.