High-pressure microscopy for manipulating cellular architecture

Abstract

Water is the most abundant substance in cells. Most intracellular reactions occur with the association of water molecules that surround the protein molecules. Application of pressure is a powerful method for modulating intermolecular interactions between protein and water molecules. This means that applied pressure is able to modulate the structure and function of protein molecules, without requiring the use of any chemical materials other than water molecules. We have developed a microscope that enables us to acquire high-resolution microscopic images at high-pressure conditions. The developed system allows us to visualize and manipulate the cellular architecture and activity.