Effect of micro ridges on single cell

Abstract

Study of the behavior of the cell by using micropattern is studied now, but it often target one kindof cell. Therefore the comparison of the cell of other kinds is difficult. It is insufficient only by control of the size same as an organization, and the control of the single cell's behavior is necessary for the application to regenerative medicine to make the cellular tissue which is near to the in vivo organization. Three types stripe micropatterns are made to control the extension direction of the cell in one direction by using Photolithography in this study. The manufactured micropatterns has mountain width, 1μm, 3μm, 5μm, height, 1μm, sulcular width, 3μm. The cell's behavior is observed by time lapse photography for 24 hours. About the extension, myoblast and fibroblast were similar results, but neuroblast was different results and confirmed the cell's behavior difference by the size of the groove width.