The effects of the mechanical deformation and trapping of the nucleus on the cellular functions using a micro fabricated-substrate

Abstract

Cells sense external forces and then translate them into biochemical signals that induce various responses. The nucleus itself has been proposed to act as a cellular mechanosensor, and the changes in nuclear shape or its deformation possibly affect the regulation of cell functions. Here we investigated the effects of deformation and trapping of the nucleus on the cellular functions using a PDMS-based micro fabricated substrates with an array of micropillars. Cells spread normally in the space between the micropillars and showed remarkable deformation of their nuclei. Such mechanical trapping of the nuclei inhibited cell migration and DNA synthesis. Interestingly, the mechanical trapping of the nuclei also inhibited the DNA damage in the ultraviolet irradiated cells. These results indicate that mechanical deformation of the nucleus using the micro fabricated substrate would be a powerful tool for regulating many cell functions.