グラヤノトキシンの培養筋肉収縮抑制効果に関する研究

抄録

Recently, research of the regenerative medicine has attracted much attention and culture techniques of the muscle cell (C2C12) have also been progressed. In this study, effect of Grayanotoxins (GTXs) to the cultured myoblast C2C12 from mouse skeletal muscle is investigated. Grayanotoxins (GTXs) belong to neurotoxins are known to enhance the permeability of a cell membrane for Na⁺ ions. It is famous as a phytotoxin, and is extracted from several kinds of Azalea family of plants such as Asebi and Azalea. As a result of a experiment, a change in a pattern of the muscle contraction, caused by an administration of GTX, has been revealed. We confirmed it depends on GTX's variety and concentration. Also, we confirmed that a high concentration 1.5-seco-GTX caused a sharp decrease of the muscle contraction.