2017 Volume 55Annual Issue 3AM-Abstract Pages 140
In order to understand the condition of mild brain injury caused by a blast wave, a technique that can identify a mild brain lesions becomes required. We established a fluorescence imaging method of the brain that underwent a clearing (transparency) treatment of fluorescent neutrophil-expressing mice for evaluation of mild brain injury. We loaded blast waves on fluorescent neutrophil-expressing mice. After extracting the brains, the clearing treatment was carried out based on CUBIC method. Then, neutrophil imaging was performed by fluorescence microscope. We succeeded in constructing a 3D image of neutrophils' distribution of the injured brain. This study revealed that an increase in the neutrophil density at the injury site was consequently visualized. This imaging method enables us to understand microbrain damage accurately and quantitatively, and to visualize of the sequential transition of brain damage and recovery.
