Measurement of the action potential of the nerve for the isolation of the nerve in the operative field: An experimental study in rabbit

Abstract

Background: Intraoperative neuromonitoring has been introduced to such surgery as head and neck, however, it is insufficient to predict the nerve function. In this study, we evaluate the nerve function by measuring the action potential directory in the operational field. Methods: Sciatic nerves of the rabbits were pulse stimulated electrically. The amplitude of the action potential of the nerve was evaluated on the nerve or surrounding tissue using by a custom-designed bio-amplifier. Results: Two different waveforms of electrotonic spread and the saltatory conduction were recorded. The conduction velocity was 60 m/s. The amplitude of saltatory conduction on the nerve and surrounding tissue 5 and 10 mm vertically away from the nerve was 2043, 891 and 562 mV. Discussion: The saltatory conduction in the neighboring tissue was evaluated. It makes possible to detect the action potential derived from the nerve within 10 mm away from the nerve in the operative field.