Effect of mechanical stimuli on chondrogenic differentiation of ATDC5

Abstract

The final goal of this study was to establish the methodology for chondrogenic differentiation of stem cells using mechanical stimuli. In this study, we evaluated the effects of cyclic compressive stimuli on chondrogenesis of ATDC5 cells cultured in agarose gel. The ATDC5 cell-seeded agarose gels were cultured under cyclic compressive stimuli (1Hz, 10% compressive strain). Cell proliferation and sulfated glycosaminoglycan (sGAG) content were quantified to evaluate the chondrogenic differentiation of ATDC5 cells. As the results, the compressive stimuli promoted the chondrogenic differentiation of ATDC5 cells using the compressive stimuli.