Improved oxygen environment reaction system adding red blood cells for a photosensitization reaction study in vitro

Abstract

To obtain sufficient oxygen dissolute solution with perceiving oxygen environment and photosensitizer bleaching in a well for photosensitization reaction study, we applied a red blood cell suspension in the well and transmission absorption spectroscopy measurement. Red blood cells suspension medium containing talaporfin sodium as a photosensitizer in a cuvette with 1 mm optical path length was employed as an optical sample. The optical sample was irradiated by a 663 nm wavelength diode laser. Transmission visible absorption spectrum of the sample were measured before and after each irradiation condition. The spectroscopy was selected to distinguish oxygenated hemoglobin, deoxygenated hemoglobin, and met hemoglobin. And also to investigate photosensitizer bleaching using Q band absorption peak. Each hemoglobin concentration was estimated using a multiple regression analysis to the obtained absorption spectrum. Oxygen saturation and absorbance peak in the Q band decrease with increasing radiant exposure were revealed by our proposing measuring method.