2017 Volume 55Annual Issue 5AM-Abstract Pages 393
Adipocyte diameter is one of the very important parameters representing adipocyte function. We newly developed a highly accurate and low-cost method for the measurement. Using rat's adipose tissue as a sample tissue, adipocytes were isolated with collagenase, fixed in formalin, and stained with oil red O. In this stage, droplets of spillage oil leaked from damaged adipocytes were hardly distinguishable from adipocytes because both shapes are very close to sphere. Therefore, we poured colorless liquid oil to wash out and lighten the red color of oil droplet only, and then adipocytes in light colored oil were suspended in water. After small amount of chloroform was added to sink and align them in monolayer to avoid defocus, clear microscopic images were captured on a PC, and cells were accurately and automatically detected as red circles to determine their diameters at once.
