2017 Volume 55Annual Issue 5AM-Abstract Pages 406
While sonodynamic therapy is a promising double-targeting cancer therapeutic, its mechanisms are still unclear. We hypothesized that cytotoxic effect of sonodynamic therapy is due to sensitizer radicals generated by electron transfers from cavitation-induced hydroxyl radicals to dye-sensitizers. Carbon-centered radicals derived from dye-sensitizer is expected to proceed oxidation of cellular membrane with high efficiency than hydroxyl radicals because those have longer lifetime than hydroxyl radicals. Here we demonstrated that cytotoxic effect of AAPH derived oxygen-centered radicals to cultured tumour cells was enhanced by presence of Ce6, a typical well-known dye for photo and sonodynamic therapy. In vitro experiments showed that cytotoxic efficacy of 100 mM AAPH was enhanced more than 20-fold in presence of Ce6. Furthermore, phthalocyanine tetrasulfonate, a photosensitizer that possess electronically stable chemical structure, did not enhance cytotoxic efficacy of AAPH. As a future work, we will investigate whether cytotoxic effect by ultrasound exposure is similar trend as AAPH.
